Rate constants for the five folding transitions act on timescales from submillisecond to tens of seconds. Subsequently, specific divalent ions modulate populations of intermediates in conformational ensembles along the folding pathway with transition times longer than 10 msec. Immediately upon addition of each divalent ion, the RNA undergoes a transition from an extended state with secondary structure to a more compact structure. coli rRNA GTPase center, we combined stopped-flow fluorescence in the presence of Mg 2+, Ca 2+, or Sr 2+ together with time-resolved small angle X-ray scattering (SAXS) in the presence of Mg 2+ to observe the folding process. To measure how different divalent cations modify folding kinetics of the 60 nucleotide E. Folding of an RNA from secondary to tertiary structure often depends on divalent ions for efficient electrostatic charge screening (nonspecific association) or binding (specific association).
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